Primary structure of carboxypeptidase II from malted barley
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چکیده
منابع مشابه
PRIMARY STRUCTURE AND ENZYMATIC PROPERTIES OF CARBOXYPEPTIDASE II FROM WHEAT BRAN by
Wheat carboxypeptidase II has been isolated from wheat bran by affinity chromatography and its enzymatic properties and amino acid sequence has been determined. The enzyme is a dimer of molecular weight around 110,000 with each monomer composed of two peptide chains, an A-chain and a B-chain, linked by disulfide bridges. The A-chain exists in two forms, one, the A'-chain, being three amino acid...
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Activity measurements and Northern blot hybridizations were used to study the temporal and spatial expression of carboxypeptidase I in germinating grains of barley (Hordeum vulgare L. cv Himalaya). In the resting grain no carboxypeptidase I activity was found in the aleurone layer, scutellum, or starchy endosperm. During germination high levels of enzyme activity appeared in the scutellum and i...
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Activity measurements and Northem blot hybridizations were used to study the temporal and spatial expression of carboxypeptidase I in germinating grains of barley (Hordeum vulgare L. cv Himalaya). In the resting grain no carboxypeptidase I activity was found in the aleurone layer, scutellum, or starchy endosperm. During germination high levels of enzyme activity appeared in the scutellum and in...
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Structural fluctuations of the apoenzyme form of carboxypeptidase A (EC 3.4.12.2) have been evaluated on the basis of molecular dynamics. The Konnert-Hendrickson refined coordinates of 2437 non-hydrogen atoms of the 307 amino acid residues derived from the X-ray structure of the holoenzyme served as the molecular model together with 548 calculated polar hydrogen atoms and 25 buried solvent mole...
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Real degree of fermentation (RDF) is an important measure of brewhouse performance in the production of beer. In this work, the relationship of RDF with malted barley starch properties was investigated. Starches were isolated from 25 malted barley samples and analyzed for molecular size distribution (by HPSEC), AP structural features (by high-performance size exclusion chromatography with multi...
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ژورنال
عنوان ژورنال: Carlsberg Research Communications
سال: 1987
ISSN: 0105-1938
DOI: 10.1007/bf02907171